Histology laboratories take a fundamental part in medical and biological research. Its importance is due to the possibility of studying cells and tissues of plants and animals. Histological studies may be conducted using tissue culture, where live human or animal cells are isolated and maintained in an artificial environment for various research projects. There are several steps to follow when it comes to preparing a sample, including fixing, processing, embedding, sectioning and staining.
In histology labs, a very common procedure is the tissue sampling, processing and staining. This is a very common and delicate process that needs to be done in the proper way, in order to not compromise the lab results. Tissue processing is about the diffusion of substances into and out of porous tissues. The diffusion process happens when you process reagents to equal concentrations inside and outside tissue blocks.
When working in any kind of laboratory, you should know that you must take some precautions, especially when working with delicate samples with acids, heating devices and other hazardous procedures. That is why recommend you to follow these following precautions when working with a hot plate in your workspace.
First, when you are heating material in a bath, make sure the glassware is heat resistant. In addition, you should inspect the glassware for cracks visible to the naked eye. Never place a glass flask, soft glass, or jars directly on a hot plate, and make sure the surface of the hot plate is larger than the object being heated. Secondly, when you are bringing liquids to a boil, adding boiling stones will help facilitate the process. In third place, you should be careful when condensing the material in a vessel until it is completely dry. If there is too little moisture and the vessel remains exposed to heat, it will eventually crack. Moreover, it is a good idea for liquids, including water, to use either the medium or medium high setting. Low boiling liquids should not be heated at the high setting, which can produce surface temperatures as high as 540 C (1004 F).
Another recommendation is that it is advisable not to heat a metal pan on a hot plate, which can damage the hot plate and maybe even pose a shock risk. In addition, when removing objects from a hot plate use tongs or rubber coated, heat resistant gripping devices. Another important recommendation is that you should not store volatile or flammable materials near a hot plate. Moreover, you should limit the use of older hot plates for flammable materials. In addition, you should check for corrosion of thermostats, which can create a spark hazard. Finally, and perhaps the most important thing of all is to remember to turn the hot plate off. Hot plates that have been left on are the source of most hot plate related injuries.
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