A Homogenizer is a simple device that is commonly used in laboratories to mix substances. It consists of an electric motor with the drive shaft oriented vertically and connected to a cup-shaped piece of rubber mounted slightly outside the center. The engine of the rubber piece swings rapidly in a circular motion. When a test tube or other appropriate container is pressed into the rubber cup (or touched to its edge) the movement is transmitted to the liquid inside and a vortex is created. Most vortex mixers have a variable speed setting and can be set to run continuously, or to run only when pressure is applied down to the rubber part.
It is an ideal instrument to gently mix and achieve a forceful resuspension of cells and chemical and biological liquid components in tubes by using an eccentric mechanism. They usually have two modes of operation:
- Continuous operation.
- Pulse operation (activated by pressing the plug with the bottom of the tube).
Vibration homogenizers are quite common in bioscience laboratories. In cell culture and microbiology laboratories, that can be used to suspend cells. In a biochemical or analytical laboratory, that can be used to mix the reagents of an assay or to mix an experimental sample and a diluent.
History
The vortex mixer or homogenizer was invented by the Kraft brothers (Jack A. Kraft and Harold D. Kraft) while working for Scientific Industries (a manufacturer of laboratory equipment). The patent was filed by the Kraft brothers on April 6, 1959 and granted on October 30, 1962. The company continues to manufacture an original version of this vortex mixer.
How should your installation be?
Place the agitator on a stable, level surface near a grounded electrical outlet. The surface should be clean and free of dust to ensure that the feet grip firmly to the surface. Leave enough free space on all sides of the unit for proper ventilation. With the switch in the OFF position, connect the power cord to a grounded outlet.
The following precautions should be observed when using or working near a homogenizer
- Never put the unit into operation if the stirring head is not firmly fixed.
- Always wear unbreakable eye protection.
- Do not use or mix flammable solvents on or near the agitator.
- Agitate hazardous samples in appropriate retention containers.
- Repairs must be carried out by qualified personnel.
- Protection deterioration may be caused if used in a manner not specified by the manufacturer.
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