In a laboratory of histology different procedures, treatments, preparation of tissues and solutions are performed, as well as measurements and treatment of images, various techniques of staining of histological samples, this service of activities requires a protocol and equipment of the workspace to develop the protocols required by researchers; in this case, water baths, are essential equipment in these laboratories.
Due to the process they perform in terms of tissues, where cuts are made thanks to the microtome, macroscopy, these successive cuts cause the tissues to be shrunk which prevents direct placement in the object holder, in this case involves the water bath or flotation bath of tissues, which has the function of extending the ribbon of tissue to get it to adhere to the lamellar.
Fabric Water Bath
These equipment are constituted by a tank made of stainless material, depends on the manufacturer has certain characteristics that can differentiate them from each other and according to the models, however, in general the water or flotation baths have a set of electrical resistors located under the tank which transfer heat to a medium such as water or oil, depends on the model and its utility, another important aspect is that they manage to maintain a preselected constant temperature.
These fabric water baths or flotation baths, inside the tank is placed a substance such as: water, unflavored gelatin, oil; where the slats of the tissues are immersed in order to extend them and then be able to fish them and attach them to the object holder, it is worth noting that to prevent the tissue from breaking it is recommended to place the water bath on a fixed table and near the microtome.
Features of fabric water baths
The characteristics of this equipment can vary between manufacturers and models, we at KALSTEIN offer you a high quality water bath with keyboard control, automatic micro computer memory and digital tube display, fast heating, long service life and energy saving, due to the use of a new type heating element; both real and predefined temperatures are shown; automatic memory and restoration functions: After commissioning, all preset temperature data are automatically stored in the system; special black surface material characterized by its strong resistance to abrasion and corrosion.
It has a container containing hot water to incubate samples in water at constant temperature for a prolonged period, has an interface that allows the user to set the appropriate temperature to achieve the desired results, in this case in the area of histology is: heating of reagents, fusion of substrates or incubation of cell cultures.
Recommendations for the use of water baths in histology
There are some recommendations for use:
- It is recommended not to use the water bath with pyrophoric or moisture sensitive reactions.
- You should constantly monitor the water level and only use distilled water; to prevent salt from being deposited in the heater.
- If your application involves fluids that emit fumes, we recommend that you operate the water bath in an extractor hood or in a well ventilated area.
- You can raise the temperature to 90 ° C or more once a week for half an hour to decontaminate the device.
- We recommend placing the water bath on a stable surface away from flammable materials.
Recommendations for the process of immersing the sheets in the flotation bath
- Place the paraffin sheets on the surface of the water, with a gentle sweeping motion.
- To eliminate bubbles that may be under the foils, make light left-to-right movements.
- Examine each section as it floats on the surface of the water, to detect possible imperfections.
- Leave the sectioned sample on the surface of the water long enough to smooth.
- To allow efficient drainage and to prevent the section from falling off the slide, remove the slides from the water in an upright position.
- Remove the remnants of lamellae inside the flotation bath by using the ends of the observing paper.
- We should not place sheets of different paraffin blocks, as either can be contaminated directly or indirectly.
If you want to buy any of the water baths or flotation tissues of histology laboratories, please be aware to contact the best, this service we can offer you through our online channels and at the best PRICE in the market, also if you want to know the catalog of high-end products that KALSTEIN have for you visit us HERE we assure you that through our online PURCHASE channels that are very easy and viable from anywhere in the world, reminding them that we are a manufacturer of high-level laboratory equipment for sale.