For the diagnosis of infections located in sterile body fluids, such as cerebrospinal fluid (CSF), pleural, peritoneal or articular fluid, the application of cytocentrifuge in histology laboratories is designed for the grouping of biological samples, providing a rapid identification of microorganisms, allowing the detection of cells, visualize the parameters of speed and time.
In this way, the equipment is very useful microbiological discipline. This technique reduces the amount of sample needed to centrifuge and obtain uniformly concentrated preparations in an area of 6 mm in diameter, increasing the performance of the technique and sensitivity in bacterial and cellular observation.
Origin of the Cytocentrifuge as Laboratory Equipment
Originally, in 1966, scientist Watson P., described the first cytocentrifuge, called as, “a device for concentrating suspended cells on a microscope slide,” and was patented in the 1980s.
Examination of cells in body fluids was historically done using a camera designed to count cells microscopically. This technique was limited by the small difference between cell types and the low number of cells present in the body fluids not concentrated. In addition, this technique did not result in permanent registration of the sample.
Currently, a cytocentrifuge is used for laboratory, designed for in vitro diagnosis (IVD). Its construction ensures simple operation and safe work. It works to separate body fluids into simple components as a result of the action of centrifugal force. It is known as a specialized technique to study cells in samples of fluid on a microscope slide, so that they can be stained and examined. They are also used in various areas of the clinical laboratory, such as cytopathology, hematology and microbiology, as well as in biological research. It can be used in a variety of samples, including fine-needle aspirates, cerebrospinal fluid, serous, synovial fluid, and urine.
Applications and use of the Cytocentrifuge
For testing, the computer is secured with a lid that can be locked because it will malfunction if the lid is kept open. Then, a sample set is integrated into the funnel in front of a microscope slide, which can be stained, and being in contact with it, is covered with filter paper, to absorb the excess liquid. Also, a few drops of fluid are placed inside the cytocentrifuge, operating with a low force (600 to 800 xg), to preserve the cell structure. Immediately, the team pushes the liquid, through the opening of the funnel and concentrates the cells in a small area of the slide, in this way, the centrifugation process concentrates the cells, approximately twenty times and creates a monolayer of a cell of thickness, which allows the evaluation of the cell morphology.
Some applications of cytocentrifuges include:
- Doing differential cell counts in body fluids, such as serous, synovial, and cerebrospinal fluid.
- Cytopathologic examination of fluid samples, such as body fluids and fine-needle aspirates.
- Gram stain of urine, liquid specimens for identification of organisms.
Kalstein-branded centrifuge
At Kalstein, we are able to meet the demands of our users in the selection of laboratory equipment. We offer you, the YR Series Centrifuge, which has attractive features such as: Microcomputer control, brushless DC motor, stable operation, low noise and high speed precision. Touch panel, programmable operation, operation data can be configured according to demands and are stored automatically. LED display, easy to use interface, simple and convenient operations. Real-time conversation of Speed and RCF is convenient for operation. It has self-locking device, lid security device, over-speed security device and automatic alarm device, etc. Silastic sealing ring comfort according to GMP. Certified by the U.S. FDA.
To learn more about our products, visit HERE At Kalstein, as manufacturers, we provide all the advice our customers need, so that their purchase is ideal, and at excellent prices. Come visit us at HERE
