Lichen platinum is a benign disease with clinical and histopathological findings. It has a clear result on epithelial transition and immune cell function. And it is detected by tests like indirect immunoflourescence Elisa which is detected with circulating antibodies in serum that bind to collagen domains.
In addition, according to the functions of the microplate washer, it will allow the detection of this disease, since it is an equipment in charge of maintaining the wells where the samples are deposited and decreasing the background character caused by the unattached antibodies, which would cause the increase of the crossovers of the analysis.
Thus, with the Kalstein microplate washer, it guarantees that only specific binding mixtures are retained, generating transmissions in the final step. It does not allow inadequate washing and avoids mismatches thanks to its adjustable and programmable washing modes.
Using the Microplate Washer in front of the Liquen Plat
In order to demonstrate the degree of danger to the health of the patients in the determination of diseases such as flat Liquen, the Elisa tests are part of these practices, since it has been demonstrated that saliva can be used as an alternative to serum for these techniques and monitoring of the disease. Therefore, with such a study technique accompanied by a microplate washer, it is able to demonstrate that symptomatic treatment options can be addressed, monitor the problem and resolve the problem of malignant transformation.
Depending on the importance of the microplate washer, the cleaning processes of the wells containing the samples and a series of measures that affect the activity of the washing passage of an Elisa plate are involved. These measures concern the aspiration and the position of the aspiration where they can be adjusted to reduce the residual volume and transitions.
The residual substances have free antibodies that enlarge the background indication, but the lower the volume of the substances, the better it is, in this way not to interfere with the next step. Also, the height of the aspiration can cause the residual volume, because if the distance between the wash tip and the bottom of the reaction well is greater, it will be enlarged in a vigorous way.
Important Features
Generally, the microplate washer uses two heads for washing processes, they are rigid and floating. The floating suction head can be easily shaken up and down during the processes, while the rigid head is more difficult to improve, and fits at an appropriate height for suction.
The position of the suction is also an important feature, as its effect is very important on the residual volume, using a single suction point per well, this is optimized and improves the function. The location depends on the structure of the head and is hardly located in the middle of the reaction well, although it is the most common default location for all washes.
Another important issue is the shape of the wells, as it affects the amount of residual material. C-shaped, flat-bottomed microplates with rounded edges are generally indicated, as they support precise amounts than those with flat bottoms and sharp corners.
Washing machine Microplate brand Kalstein
Kalstein, meets the programs of soaking time and vibration, in addition to having a power supply of 220 – 110 VAC ± 10%, 50 60Hz 100W. Automatic waste and lotion and distilled water liquid warning. As well as precision dosing Cv <2% for multiple laboratory practices.
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