A fluorometer is a laboratory instrument that enables the analysis, identification and quantification of chemical substances with molecules capable of exhibiting fluorescence, using the technique of fluorescence spectrophotometry or fluorometry.

This equipment is a special optical device capable of measuring the fluorescent quality of biological or mineral samples. Fluorescence is a type of luminescence that occurs in certain substances, giving them the quality of being able to absorb electromagnetic radiation (light), and emit some of this energy in the form of radiation at a different wavelength to that absorbed.

Structure of a fluorometer

A fluorometer is made up of 4 main components: the radiation source (Xenon lamp, or laser, in addition to other lamps that can cover a wider spectrum of wavelengths), an excitation monochromator (which selects the appropriate wavelength for sample excitation), an emission monochromator (which analyzes the emissions produced by the fluorescence emitted by the sample) and a photomultiplier, which receives the light waves and transforms them into a measurable unit (number), which is going to be directly related to the intensity of the emitted light.

Fluorometer care and recommendations

• Clean computer surface.
• Clean filters and light sources.
• Check electrical installations.
• Test equipment operation: switch on equipment and let it warm up for about 15 minutes, select the wavelength to be used, select the function, reset with distilled water.
• Place the equipment in a place free from vibration, excessive heat, humidity or direct light.
• Protect your computer from dust. Do not touch optical components such as lenses and filters directly. Follow the manufacturer’s instructions for cleaning these components.
• Allow the equipment to warm up before taking any measurements.
• Periodically check wavelength adjustment when there is reason to suspect that it has changed.
• Make sure that reading trays are clean, free of scratches and fingerprints.

Among the applications where a fluorometer is used, we can mention the following:

• Protein dynamics
• Quantitative analysis
• Cell membrane studies
• Enzyme kinetics
• Carbon nanostructures
• Characterization of fluorescent probes
• Short-time phosphorescence measurements
• Thermal stability measurements

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