Real-time thermal cyclers
A real-time thermal cycler is a laboratory equipment that allows the real-time polymerase chain reaction (qPCR) to be carried out efficiently and quickly; by means of the automatic and cyclical realization of the temperature changes that are required for the amplification of a deoxyribonucleic acid (DNA) chain; from a thermostable enzyme. The objective of this type of PCR is to detect and quantify the specific nucleic acid sequences by means of fluorescent molecules in the reaction, where the increase in fluorescence is proportional to the increase in the amount of amplified DNA molecules in the reaction.