A laminar flow booth is laboratory equipment that provides an area bounded by surfaces that are easy to clean and disinfect with a flow of filtered air through pre-filters, which are responsible for retaining the larger particles that are present in it. air, and by HEPA filters (High Efficiency Particulate air), which are the high efficiency filter capable of retaining particles down to 0.3 um with a minimum efficiency of 99.97% providing clean air and constant at a rate of 0.3 to 0.5 meters per second .. That is, this type of equipment is used in processes where contamination control is required.
In other words, it can be said that a laminar flow bell, creates a particle-free environment, by taking air through a filtration system and ejecting it through the work surface by means of a flow. laminar or unidirectional air.
This is why laminar flow booths are used for microbiological control procedures; packaging process, sterile filling; But they should not be used when working with strains of polluting biological agents.
The laminar flow cabin is closed on the sides and maintained under constant positive pressure to prevent the infiltration of contaminated air from the environment. It generates « air barriers », allowing air to flow at a constant speed in one direction, something which is also known as laminar air flow.
How does a laminar flow bell work?
Laminar flow bells maintain the recirculation of clean air in the environment where they are located; Continuous air renewal. They take from their environment; They filter it and return it to the environment with an additional step of absolute filtration, and this process is constant.
In laminar flow booths, the air outlet speed, constantly, continuous and homogeneous through the distribution of the HEPA filters, allows the particles generated in the work area to be moved, causing a sweeping effect out of this area. .
General Considerations for Working in Laminar Flux Cabins
- This equipment must be placed in neighborhoods, free of drafts, and restricted access. There is usually a previous cloakroom where the staff is placed the dress and other clothing.
- The personnel who will work in the bell must wear clean clothes. Long dresses are recommended with closed fists, hats and blankets. Depending on the task at hand, the use of gloves may be necessary (without talcum powder). In other cases, the use of special sterile uniforms is necessary.
- Unidirectional air flow can be disturbed by drafts caused by sudden movements made inside, by walking quickly in front or by teams that can produce them, for which it is recommended not to introduce and to pull their hands out while carrying out the work and move them slowly inside.
What should you do when working in a laminar flow booth?
- Make a list of all the materials that will be needed to complete the job.
- Disinfect the work surface with a cloth dampened in 70% alcohol from back to back, repeating the process with overlapping motions.
- Place all the materials in the order they will be used.
- Put the bell or cabinet on for at least 30 minutes before starting to work.
- Before entering the area where the bell or cabinet is located, wash your hands and forearms with a soap that has antimicrobial activity. Dry them with rags that release a low number of particles.
- Remove all equipment materials at the end of the job, with disinfect
70% alcohol as indicated above.
- Leave the bell on for at least 15 minutes so that any contaminants can be removed from the work area.