Most dyes used to visualize the membranes and organelles of the cell are water-soluble. The embedded wax must therefore be removed prior to staining. This is done by effectively reversing the tissue-processing schedule.
The staining process is a routine technique that reveals exceptional detail of tissue structure and makeup of the cells. It is a very common auxiliary technique in the microscopy field. In the staining process, the operator highlights the structures in biological tissues and that allows a more detailed look of the sample.