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Use of Elisa scrubber to assess cytotoxicity of chemotherapeutic medicinal products

The micro-plate washer is laboratory equipment used to perform mechanical washing operations directly to the 96 or 384 well blades used for chemical research. They are widely used to maintain the hygienic sample deposition instrument during research, drug discovery, and quality control.

In this case, for the assessment of the degree of toxicity of chemotherapeutic medicinal products, the microplate reader analyzes all compound levels or the effect of cytokines on cells, where DNA analysis is required and using reproduction tests. This requires microplate washing machines with key processes for the proper conduct of the study, through the different washing steps you can achieve to eliminate all kinds of residue that has remained free without reacting, preventing unwanted interference.

Operation of the Elisa washer

The micro-plate washer is an injection and suction equipment that automatically extracts and dispenses liquids to clean the micro-plates, removing any substance that remains adherent when using the Elisa technique. It has a suitable space where the washing pad is placed and mechanically rinsed for 6 minutes. Then, the user, after scheduling the cleaning processes, should place the plate upside down and shake it firmly on a paper towel to remove any excess.

The micro-plate washer has two tanks, in one it places the washing pad while in the other it collects the substances that are thrown away during the Elisa technique. It has control, procurement, extraction and advancement mechanisms.

It also offers the ability to control settings according to the specific requirements of each cleaning system, and also for the permanent recording of these tests, stored in the internal memory of the computer that can be selected from the menu that manages its functions.

General process of the Elisa technique

First, the importance of the enzyme immunoassay test leads to studies to determine the efficacy and specificity of the antigens tested, to be captured by a monoclonal antibody that is attached to the well surface of the microtitre plate. The quantity retained in the wells is evaluated and the intensity of the color of the sample is measured. The functionality of the Elisa technique is confirmed by positive processes such as antigens, as well as by negative control of antigen-free buffer solutions, used to normalize patient sample results and evaluate toxic levels that influence chemotherapeutic drugs, which could give high false values.

In this way, the Elisa washer works to ensure that the results are reliable and accurate. Due to their importance, correct steps should be performed and interruptions should be avoided, such as:

  • Verify that the quantity dispensed into the wells.
  • Check the effectiveness of the suction system.
  • Needles must be clean for supply and extraction processes.
  • Check the cleaner body.
  • Check control settings.

Microplate Washing Machine (Elisa) brand Kalstein

The micro-plate washer, become the best ally for laboratory testing, and in Kalstein we have the best lines in equipment, able to meet all the needs of our users, moreover, as manufacturers we offer you the ideal equipment belonging to the YR series, which contains a 96 well head. Removal of a single needle, no suction needle, no blocking. The washing time is less than 30 seconds for a dish. Among other features you can view from HERE

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